Herein we report an optimization study of various phycocyanin extraction methods from spirulina platensis cyanobacterium biomass dry, frozen and wet. Cphycocyanin extraction from spirulina platensis wet biomass. A simple method for efficient extraction and purification of. Extraction, application, and challenges concerning production stephen bleakley 1,2 and maria hayes 1, 1 food biosciences department, teagasc ashtown food research centre, ashtown, dublin d15 kn3k, ireland. Phycocyanin is an abundant proteinpigment complex in many photosynthetic microbes and the feedstock for several emerging products in the food and pharmaceutical industries. Phycocyanin being a very high value product, enhanced recovery from the residual biomass is highly desirable. Spirulina platensis was developed by a hexane extraction process. Therefore phycocyanin extraction from fresh biomass was studied. Therefore fresh biomass was suitable for phycocyanin extraction. If you are unable to see the pdf link please follow the. Ravishankar plant cell biotechnology department, central food technological research institute, mysore 570 0, india.
Extraction and purification of c phycocyanin from dry spirulina powder and evaluating its antioxidant, anticoagulation and. The extractions were carried out using two different methods. In all the strains tested the extraction efficiency proved to match what reported in literature while in regards of the purity all the strain achieved higher values arthrospira platensis 100%, rhodomonas sp. The invention relates to an extraction method of spirulina phycocyanin. Extraction and separation of phycocyanin from spirulina using. Although a number of reports are available for the extraction and purification of phycocyanin from cyanobacterial strains schoenleber et al. Spirulina is used as a high quality protein mainly for phycocyanin eriksen 2008, which is a important cyanobacterial accessory pigment having a number of industrial applications. Methods for extraction, isolation and purification of cphycocyanin.
Stable isolation of phycocyanin from spirulina platensis associated with highpressure extraction process. Phycocyanin extraction the extraction method is developed based on the previous publication 8. A number of drying methods studied for the processing of spirulina oven dried and sun dried resulted in loss of phycocyanin. There are some difficulties in phycocyanin extraction because of multilayered cell walls and large amounts of contaminants. Improving the biomass productivity and phycocyanin concentration by mixotrophic cultivation of arthrospira platensis. Oct 27, 2018 sarada r, pillai mg, ravishankar ga 1999 phycocyanin from spirulina sp. Membrane processes was used in this study tion in phosphate buffer ph 7. Considering the uses of cphycocyanin, the aim of this study was to evaluate some methods previously reported to extract cphycocyanin and other bioproducts, in order to find the best procedure to extract cphycocyanin from wet biomass of spirulina platensis considering the extraction yield. Qucccm 56 under different irradiance and temperature. A wide range of phycocyanin extraction methods were studied for the processing of. The purification yield of this method was estimated as 10. The optimized concentration and purity of spirulina.
Results showed that only the extraction time significantly affected p isolation and purification of cphycocyanin have been studied for many years, resulting in diverse methodologies with a range of yields and grades of purity. Phycocyanin concentration is used as a cyanobacteria monitoring tool, utilizing phycocyanin to chlorophyll a ratio as a cyanobacteria bloom condition indicator. Extraction and purification of phycocyanin from microalgae has been achieved by different ways and can be categorized under two methods. Ijms free fulltext stable isolation of phycocyanin. The objective of this study was to isolate phycocyanin from dried biomass of spirulina platensis. Two gram of biomass was suspended in 30, 50, 75, or 100 ml of water in a 250ml baffled shake flask the flask was placed in incubator shaker heidolph incubator, schwabach, germany that run at 25c figure 1 for 30.
Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s. Improving the biomass productivity and phycocyanin. The effects of temperature and biomasssolvent ratio on phycocyanin concentration and extract purity were evaluated to. The optimized concentration and purity of spirulina platensis. Improving the biomass productivity and phycocyanin concentration by mixotrophic cultivation of arthrospira platensis master thesis, june 2016. Of the extraction methods tested, freezing and thawing of cells, homogenisation using a mortar and pestle in the presence of abrasive material and homogenisation using. Compared to combination of lysozyme followed by high pressure 2000 psi, glass beads destruction showed high efficiency in isolation of allophycocyanin crude extract. Extraction methods for phycocyanin determination in.
Sarada r, pillai mg, ravishankar ga 1999 phycocyanin from spirulina sp. Methods for extraction, isolation and purification of c phycocyanin. Wt40 phycocyanin extraction in spirulina produced using. Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume. In particular, phycocyanin has been widely considered as. Pdf methods for extraction, isolation and purification. Jul 16, 2014 spirulina is used as a high quality protein mainly for phycocyanin eriksen 2008, which is a important cyanobacterial accessory pigment having a number of industrial applications. An improved and efficient method for the extraction of. Jan 16, 20 a method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined with high pressure. Considering both yield and purity, ultrasoundassisted extraction alone was inferred to be the most suitable method for extraction of phycocyanins from the dry biomass of a. The cost of phycocyanin production depends on the cost of spirulina cultivation.
Methods for extracting phycocyanin algenol biotech llc. Pc concentration and purity ratio were significant. Phycocyanin extraction and determination handbook of. Pdf separation and purification of phycocyanin from. Pdf extraction methods for phycocyanin determination in. A method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined with high pressure. The selection of the method to extract phycocyanin with maximum yield and highest activity antioxidant mainly depends on the nature of compounds and thermal stability for extraction. In this study, comparison effect of mechanical freezing and thawing, sonication and bead milling along with chemical inorganic acid, buffers and sea salt extraction methods were carried out on c phycocyanin concentrations to achieve the optimum conditions of phycocyanin extraction from spirulina platensis. However, hot water method was timeconsuming and achieving only a low extraction rate, while ultrasonic extraction was difficult to control and liable to induce changes in the. Extraction and characterisation of analytical grade c. Extraction methods for phycocyanin determination in freshwater filamentous cyanobacteria and their application in a shallow lake. Carotenoids, chlorophylls and phycocyanin from spirulina.
Research article phycocyanin extraction from spirulina platensis and extract stability under various ph and temperature. Several of the above phycocyanin extraction and purification methods were compared for the final e1%. However, phycocyanin may also be found in cryptophyta, glaucophyta, rhodophyta, and dinophyta. The results showed that the linear and interaction effects of two parameters of extraction time and buffer ph on c. Optimization of extraction method and characterization of. Kovacs1, caitlin riddick2 and matyas presing1 1centre for ecological research, hungarian academy of sciences, balaton limnological institute, h8237 tihany, hungary. Methods for extraction, isolation and purification of c. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural institutedeemed university, dindigul, tamilnadu, india corresponding author abstract introduction spirulina is a bluegreen algae owing to the. Cn102993297a spirulina phycocyanin and extraction method. The aim of the research was to use these two green extraction processes to obtain cpc from cyanobacteria arthrospira maxima because. Jan 25, 2020 combination of methods did not result in significant improvement with respect to yield and purity. Extraction methods for phycocyanin determination in freshwater.
This was necessary because this dye is known to be very unstable during normal extraction processes. Phyocyanin extraction from microalgae spirulina platensis. By selecting the most accurate set of equations available in spectrophotometry and by using a simple but efficient saline buffer extraction, the method proposed in this sop allows an easy, fast, and low. The extraction yield of the phycocyanin from the three different methods was estimated by the procedure below. A method for stably purifying a functional dye, phycocyanin from. Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. Benchtop fluorometry of phycocyanin as a rapid approach for. Benchtop fluorometry of phycocyanin as a rapid approach. One, wherein the cells are subjected to enzymatic disintegration. Because of these benefits, numerous researchers have focused on developing efficient processes for mass production of phycocyanin producing strains 10, 11 and extraction of phycocyanin from microalgae 5, 12.
Pdf phycocyanin extraction from spirulina platensis and. Extraction and separation of phycocyanin from spirulina. The htl biocrude oil obtained from the original spirulina feedstock and the residual after the cpc extraction were compared. The recovery of phycocyanin is normally done from fresh algal biomass where the cake collected after 1st stage of phycocyanin extraction is disposed. Evaluation of different extraction methods for phycocyanin. Though various processes for the extraction of phycocyanin are known, the extraction. In the present study, a simple and efficient method to extract cphycocyanin from spirulina platensis biomass is used. Foodgrade phycocyanin needs to have a certain amount of purity and e1%. The purpose of this project was to research the effectiveness of. Oct 25, 2018 purity and e1% value of phycocyanin prepared by various methods. Rapid green extractions of cphycocyanin from arthrospira. Comparison of different extraction methods for phycocyanin. Pdf methods for extraction, isolation and purification of c. The optimum extraction was through freezethawing extraction with 0.
Optimization of phycocyanin extraction from spirulina. Maximising phycocyanin extraction from a newly identified. Phyocyanin extraction from microalgae spirulina platensis assisted by ultrasound irradiation. Combination of methods did not result in significant improvement with respect to yield and purity. Extraction methods for phycocyanin determination in freshwater filamentous cyanobacteria and their application in a shallow lake article pdf available in european journal of phycology 483. The extractions were carried out using six different. Measurement of extraction yield and the purity of phycocyanin. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a. In this study, the extraction of phycocyanin from wet biomass figure 1. Phycocyanin is an important commercially available blue food colorant. Because of these benefits, numerous researchers have focused on developing efficient processes for mass production of phycocyaninproducing strains 10, 11 and extraction of phycocyanin from microalgae 5, 12.
Table 1 below lists the purity information for the various samples. Several methods have been reported for successful separation of phycocyanin 17 but these methods comprised multiple steps and are time consuming. A number of drying methods studied for the processing of spirulina crossflow dried, spray dried and oven dried resulted in approximately 50% loss of phycocyanin. Briefly, cpc was extracted from spirulina platensis dry powder 1. B four photobioreactors 50 l tanks with initial volumes of 20 l of s. Study of phycocyanin production from spirulina platensis braz. A process for the extraction of phycocyanin patent details.
Previous study shows water is a good solvent for phycocyanin extraction 8. Phycocyanin extraction from cyanobacteria spirulina platensis was optimized using factorial design and response surface techniques. It also seems like there is a correlation between high biomass density and low phycocyanin content. The sonication method using an ultrasonic processor was conducted for 5, 12. In the present study, a simple and efficient method to extract cpc from spirulina platensis dry powder is reported. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing the method most frequently used, and presented a extraction yield of 43. Purity and e1% value of phycocyanin prepared by various methods. Extraction and purification of cphycocyanin from dry. A simple method for efficient extraction and purification. Study of phycocyanin production from spirulina platensis. In the last decade, the cyanobacterium spirulina has gained a high commercial interest as a food supplement, mainly due to its high protein content as well as high amounts of pigments, such as carotenoids, chlorophylls and phycocyanins. Stable isolation of phycocyanin from spirulina platensis.
An improved and efficient method for the extraction of phycobiliproteins for optimization of phycocyanin productivity in leptolyngbya sp. Oct 29, 2014 phycocyanin from spirulina sp influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin process biochem. Quantitative analysis of cphycocyanin from spirulina. Quantitative analysis of cphycocyanin from spirulina pacifica low temperature method background. Hot water method and ultrasonic extraction were among the methods of extracting phycocyanin patil and raghavarao, 2007. Spirulina arthrospira exerts a wide spectrum of pharmacological. Experiments were conducted to determine the effects of sonication time on the release of phycocyanin from concentrated s. Alternate method of phycocyanin extraction by will. Extraction and purification of cphycocyanin from spirulina. When used to augment the current methods of phycocyanin production, it has the potential benefit of reducing cell extraction volumes and increasing the yield of phycocyanin. A protocol has been designed to extract phycobiliprotein from leptolyngbya sp. Cphycocyanin was extracted from spirulina platensis with highspeed.
Purification, characterization, and activity evaluation of. The phycocyanin extract in the above method was prepared. Various methods of cell disruption, such as highpressure homogenization, ultrasound treatment, ultraturrax homogenization, and cell lysis have been applied. Optimization of phycocyanin extraction from microalgae. The mineral medium used is the largest cost component in the cultivation. Methods we performed an exhaustive search using scopus and pubmed databases to find methods for the separation, isolation and. However, cultivating with only red light did not seem to effect phycocyanin content. The process for extracting functional proteins in conjunction with phycocyanin and phycoerythrin extraction from a. The residual biomass after cpc extraction was converted into biocrude oil via hydrothermal liquefaction htl. Of the extraction methods tested, homogenisation using a mortar and pestle yielded 18.
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